Biochemistry: Enzyme Kinetics and Metabolic Regulation
Test your understanding of glycolysis thermodynamics, enzyme kinetics, and metabolic regulation through progressively challenging questions.
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Quiz Questions & Answers
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Question 1: What is the net ΔG°' value for the conversion of glucose to glucose-6-phosphate in glycolysis?
-13.8 kJ/mol
Question 2: Which wavelength is typically used to monitor NADH absorbance in LDH enzyme assays?
340 nm
Question 3: What distinguishes glucokinase from hexokinase I in terms of glucose affinity?
Glucokinase has a much higher Km (~10 mM) compared to hexokinase I (~0.1 mM)
Question 4: In the lactate to pyruvate conversion, what makes the reaction thermodynamically favorable?
The positive ΔE°' of +0.13 V between the half-reactions
Question 5: How does G6P differently affect hexokinase I versus glucokinase?
G6P inhibits hexokinase I allosterically but has no effect on glucokinase
Question 6: On a Lineweaver-Burk plot, what indicates competitive inhibition?
Lines intersecting at the y-axis
Question 7: Why is hexokinase I saturated under normal blood glucose conditions?
Its low Km (~0.1 mM) compared to normal blood glucose (5 mM)
Question 8: What cellular condition makes the glucose to G6P conversion even more favorable than standard conditions suggest?
Actual cellular concentrations drive ΔG to -32 kJ/mol
Question 9: How do MODY mutations affect glucokinase function?
They increase the Km, reducing activity at normal glucose levels
Question 10: What type of plot shows parallel lines for uncompetitive inhibition?
Eadie-Hofstee plot
Question 11: How does dorzagliatine improve glucose regulation?
It lowers glucokinase's apparent Km, enhancing its activity at lower glucose concentrations
Question 12: What parameter is calculated by dividing Vmax by total enzyme concentration [E]?
kcat (turnover number)
Question 13: Why does glucokinase's high Km prove advantageous in the liver?
It prevents futile cycling and allows response to glucose fluctuations
Question 14: In non-competitive inhibition, what parameter remains unchanged?
Km
Question 15: Why is the lactate to pyruvate conversion particularly important in the liver?
It supports gluconeogenesis during fasting by reversing glycolysis