Bacterial Staining Techniques Quiz
Test your medium-level knowledge on Gram and acid-fast staining, key methods for bacterial identification based on cell wall properties.
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Quiz Questions & Answers
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Question 1: Gram staining differentiates bacteria based on:
D. Size
C. Motility
A. Shape
B. Cell wall composition
Question 2: In a clinical lab scenario, Gram-positive bacteria appear purple because they retain:
B. Crystal violet
A. Safranin
C. Methylene blue
D. Malachite green
Question 3: The decolorizer in Gram staining, which removes stain from Gram-negative cells but not Gram-positive, is:
D. Safranin
A. Water
C. Iodine
B. Alcohol/acetone
Question 4: Acid-fast staining, used for detecting resilient pathogens like Mycobacterium, employs which primary stain?
C. Carbol fuchsin
D. Crystal violet
A. Methylene blue
B. Safranin
Question 5: Acid-fast bacteria resist decolorization in their staining procedure due to the presence of:
C. Lipopolysaccharide
D. Capsule
A. Peptidoglycan
B. Mycolic acid