Cell Culture Basics (EU)
Medium-difficulty multiple-choice quiz covering core concepts, safety, aseptic technique, contamination, culture methods, and cryopreservation from the Cell Culture Basics handbook.
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Quiz Questions & Answers
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Question 1: What best describes the difference between finite and continuous cell lines?
Finite lines are always primary isolates; continuous lines are always transformed bacteria
Finite lines require cryopreservation; continuous lines cannot be frozen
Finite lines have limited lifespans; continuous lines can proliferate indefinitely
Finite lines grow only in suspension; continuous lines grow only attached
Question 2: Which laboratory practice is most important to prevent biological contamination during routine cell culture work?
Working aseptically in a properly maintained sterile work area or hood
Incubating cultures at room temperature to slow microbial growth
Using antibiotics in every culture to mask contamination
Opening multiple incubators at once to equalize CO2
Question 3: Why is monitoring CO2 and pH important for mammalian cell culture?
CO2 sterilizes the incubator environment and reduces contamination
CO2 levels determine osmolarity of the medium
CO2 helps maintain the bicarbonate buffer system and therefore media pH
CO2 provides an energy source for adherent cells
Question 4: When subculturing adherent mammalian cells, what is the primary purpose of a dissociation reagent like TrypLE or trypsin?
To cryoprotect cells before freezing
To change the media pH rapidly prior to incubation
To break cell–substrate and cell–cell attachments so cells can be removed and replated
To permanently inactivate contaminating mycoplasma
Question 5: What is a recommended strategy in the handbook for preserving valuable cell lines long-term?
Keeping cells at room temperature with antibiotics as a backup
Culturing continuously without passaging to avoid freeze–thaw cycles
Cryopreserving early-passage cells using an appropriate freezing medium and controlled-rate cooling
Storing flasks in a standard incubator at 37 degrees Celsius
Question 6: Which contaminant is especially hard to detect visually and requires specific testing for accurate identification?
Mold
Obvious fungal hyphae on flask surfaces
Large bacterial colonies in the media
Mycoplasma
Question 7: Which statement best reflects the handbook's guidance about using antibiotics in routine cell culture?
Antibiotics eliminate mycoplasma reliably without testing
Antibiotics are mandatory in all mammalian cultures to prevent bacterial growth
Antibiotics can mask poor aseptic technique and are not a substitute for good practices
Antibiotics improve cell line genetic stability over passages
Question 8: If a cell culture shows sudden changes in morphology, growth rate, and medium appearance, what is the handbook's recommended evaluation first step?
Immediately discard all stocks without investigation
Assess for biological contamination (bacteria, yeast, mold, mycoplasma) and check aseptic handling
Add extra serum to the medium to restore morphology
Increase incubator temperature to speed recovery