Cells of the Immune System (1) — Cell Culture Basics
A medium-difficulty medical lab science quiz focused on cell culture principles and practices from the provided material.
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Quiz Questions & Answers
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Question 1: What term describes cells taken directly from tissues that can only divide a limited number of times in culture?
Primary cells
Stem cell clones
Established cell lines
Hybridomas
Question 2: Which culture type best describes cells that must attach to the flask surface to grow?
Semi-solid culture
3D organoid culture
Adherent cell culture
Suspension cell culture
Question 3: Which cell morphology is typically spherical and grows in suspension rather than attaching to surfaces?
Neuronal-like cells
Lymphoblast-like cells
Fibroblastic cells
Epithelial-like cells
Question 4: Which medium is specifically noted as complex and commonly used for culturing human lymphocytes?
RPMI-1640
DMEM
EMEM
Ham's F-12
Question 5: What is the primary purpose of cell passaging in culture laboratories?
To permanently freeze cells for storage
To sterilize the culture vessel
To measure cell morphology
To transfer cells to fresh medium and expand healthy populations
Question 6: Which additive in culture medium is highlighted for extending culture lifespan and reducing frequent passaging?
GlutaMAX
Non-essential amino acids
Antibiotics
Sodium pyruvate
Question 7: During cryopreservation, why is freezing cells considered crucial for cell line management?
It increases cell proliferation rates
It replaces the need for aseptic technique
It preserves cells long-term and protects valuable cell stocks from loss
It eliminates genetic drift in cultures
Question 8: Which of the following is NOT listed as a common source of contamination in the cell culture lab?
Fungal contamination
Mycoplasma
Prions
Bacterial contamination